• Calcium mobilization FLIPR assay CHO K cells expressing an

  2021-12-23

  

  Calcium mobilization (FLIPR) assay: CHO-K1 cells expressing an NFAT-β-lactamase reporter and the promiscuous Gα-subunit Gqi5 were stably transfected with either empty vector (pCDNA3.1, Invitrogen) or vector expressing HA-GPR81, GPR109A or GPR109B. Cells were seeded at 10,000cells/well in 384-well cu

• br Conclusions Beta cell specific expression

  2021-12-23

  

  Conclusions Beta cell-specific expression in human islets of DGCR2, GBF1, GPR44 and SerpinB10 has not previously been described, although the proteins have partly been characterized in other contexts not related to beta TCS JNK 6o or diabetes. DGCR2, GPR44 and SerpinB10 were negative in all othe

• Up to now many UDG

  2021-12-23

  

  Up to now, many UDG assays have been reported based on the design of dU-containing substrate DNA, which can be roughly divided into two categories according to the sensing mechanisms. One type of UDG assays are simply based on the physical or chemical property changes of the substrate accompanied wi

• br Methods br Results and discussion

  2021-12-23

  

  Methods Results and discussion Protein analysis of commonly used cell lines (C6, D54-MG, U87, U251 and STTG1) reveals high fluorescent probes of SXC (Cho and Bannai, 1990, Ye et al., 1999, de Groot et al., 2005, Lyons et al., 2007, Savaskan et al., 2008). Since protein expression can be alter

• br Materials and methods br Results br

  2021-12-23

  

  Materials and methods Results Discussion The glucose transporter GLUT1 can be acutely activated or inhibited in L929 fibroblast WAY 208466 dihydrochloride by a wide variety of reagents or nutrient conditions [[9], [10], [11], [12],36,50]. This abrupt change in the activity of GLUT1 occurs w

• The orientation of the immobilized enzyme

  2021-12-23

  

  The orientation of the immobilized enzyme may affect its activity and stability and therefore the final biocatalyst performance. Since proteins present areas more prone to unfolding in their surface or near to it [36], the immobilization through these areas may improve the final biocatalyst stabilit

• lumiracoxib synthesis Magtanong et al observed that

  2021-12-22

  

  Magtanong et al. (2019) observed that caspase-dependent apoptotic lipotoxicity of saturated fatty acids is suppressed by exogenous MUFAs. This may explain the ability of MUFAs to prevent atherosclerosis, in contrast to the pathogenicity of saturated fatty acids. Moreover, MUFAs may promote while PUF

• HER genotype was determined in breast cancer tissue

  2021-12-22

  

  HER2 genotype was determined in breast cancer tissue of 73 breast cancer patients. As previously reported, Ile655Val and Ala1170Pro genotypes measured in breast cancer tissues were available for 71 and 69 breast cancer patients, respectively. Briefly, 77.5% (55/71) of patients were homozygous for th

• br Conclusion In conclusion we showed that apoptosis is invo

  2021-12-22

  

  Conclusion In conclusion, we showed that apoptosis is involved in etoposide-induced pancreatic β-cell cytotoxicity. More importantly, as shown in Fig. 6, the results of this study demonstrated that etoposide induces pancreatic β-cell death via the JNK/ERK activation-mediated GSK-3 downstream-trig

• Materials and Methods br Results br Discussion Activation

  2021-12-22

  

  Materials and Methods Results Discussion Activation of TLRs by damage-associated patterns is a well-recognized trigger for inflammation. TLR ligation results in a large increase in glycolytic metabolism in macrophages and other immune cells,12, 27 and we hypothesized that the glycolytic metab

• br Gastrointestinal peptides and intestinal

  2021-12-22

  

  Gastrointestinal peptides and intestinal health Gastrointestinal peptides are intimately involved in the regulation of feeding behaviors, energy metabolism and bodyweight 36, 37, 38, 39, 40, 41. GPR120 has been demonstrated to induce secretion of two such peptides: GLP-1 and cholecystokinin (CCK)

• For several parasites of veterinary importance there is a gr

  2021-12-22

  

  For several parasites of veterinary importance, there is a growing body of evidence that resistance to ML is, at least in part, mediated by ABC transporters (P-glycoproteins) (Whittaker et al., 2017). P-glycoproteins (Pgps) are members of the ATP-binding cassette transporter family that are well kno

• br Investigations br Genetics GLUT is

  2021-12-22

  

  Investigations Genetics GLUT1 is a membrane-bound glycoprotein that provides base-rate glucose transport across blood-tissue barriers. It is constituitively expressed in erythrocytes, synthase microvessels and astroglia. The gene exclusively associated with GLUT1 is SLC2A1, located on chromos

• Given the proposed importance of

  2021-12-22

  

  Given the proposed importance of conformational dynamics to GCK's allosteric regulation, a number of biophysical methods have been utilized to probe the enzyme's dynamic structural landscape. Investigations into the mechanism of ikk inhibitor binding, using changes in the enzyme's intrinsic fluores

• As ghrelin effects are suspected to be mediated

  2021-12-22

  

  As ghrelin effects are suspected to be mediated by a heterogeneous population of G-protein-coupled-receptors [2], [21], other so far unknown subtypes of ghrelin receptors differing from the classical GHSR-1a may additionally exist that might also mediate ghrelin effects on the heart. Thus, the real

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